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  • Bacterial Regulatory RNA

    Methods and Protocols

    Series series Springer Protocols
    This second edition details new and updated methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters detail discovery of ncRNAs, characterization of their structure, functions, and their interactomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary ... Leer más

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  • Mass Spectrometry-Based Proteomics

    Edición de Kris Gevaert ...
    Series series Springer Protocols
    This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by ... Leer más

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  • Bacterial Secretion Systems

    Methods and Protocols

    Edición de Laure Journet, Eric Cascales ...
    Series series Springer Protocols
    This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or ... Leer más

    $197.99 USD

  • Mitochondrial Medicine

    Volume 2: Assessing Mitochondria

    Edición de Volkmar Weissig, Marvin Edeas ...
    Series series Springer Protocols
    This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional’ mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D ... Leer más

    $107.99 USD

  • Proteases and Cancer

    Methods and Protocols

    Edición de Salvatore Santamaria ...
    Series series Springer Protocols
    This volume details bioinformatics analysis, biochemical assays, recombinant protein expression and purification, methods to investigate protease activity in cell-based, organoids and in vivo systems, proteomics, transcriptomics, machine learning and novel approaches to target dysregulated protease activity in cancer. Written in the highly successful Methods in Molecular Biology series format, ... Leer más

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  • Lipopolysaccharide Transport

    Methods and Protocols

    Edición de Paola Sperandeo ...
    Series series Springer Protocols
    This volume provides the most widely used protocols and techniques employed in lipopolysaccharide( LPS) transport studies. Chapters detail identification and analysis of essential genes and crucial mutations in LPS transport-related genes, LPS profile determination, LPS chemical analysis, protein-LPS interaction, and LPS visualization. Written in the format of the highly successful Methods in ... Leer más

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  • Peroxisomes

    Methods and Protocols

    Edición de Michael Schrader ...
    Series series Springer Protocols
    This fully updated volume assembles a comprehensive collection of methods, techniques, and strategies to investigate the molecular and cellular biology of peroxisomes in different organisms. Peroxisome research is on the rise, as novel functions and proteins of this dynamic organelle are still being discovered through studies in model systems including humans, mice, flies, plants, fungi, and yeast ... Leer más

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  • Intracellular Lipid Transport

    Methods and Protocols

    Edición de Guillaume Drin ...
    Series series Springer Protocols
    This fully updated volume explores different classes of lipid transporters, such as lipid transfer proteins, flippases, and scramblases, and significant advances in solving their biological functions. The first half of the book features methodologies to measure the movement of lipids between or in organelle membranes inside eukaryotic cells, including plant cells, or in prokaryotic cells, and the ... Leer más

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  • Dictyostelium discoideum

    Methods and Protocols

    Edición de Alan R. Kimmel ...
    Series series Springer Protocols
    This volume discusses the latest advancements and techniques used to study Dictyostelium discoideum. The chapters in this book cover topics such as cytokinesis, membrane function and damage, and nutrient/bacterial processing; autophagy activation and function; protein localization, interaction, and activation; cell migration and contact guidance for quantification of cytoskeletal dynamics; and ... Leer más

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  • HDAC/HAT Function Assessment and Inhibitor Development

    Methods and Protocols

    Edición de Oliver H. Krämer ...
    Series series Springer Protocols
    This fully updated edition provides a series of methods for how best to assess functions of histone deacetylases and acetyltransferases. The disease-relevance of dysregulated protein deacetylation by overexpressed or aberrantly activated histone deacetylases has spurred an intense search for novel and improved inhibitors of these enzymes, as reflected in this collection. Expert contributors ... Leer más

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  • Protein Tyrosine Phosphatases

    Methods and Protocols

    Series series Springer Protocols
    This second edition volume expands on the previous edition with discussions on the latest advancements in protein tyrosine phosphatases (PTP) research used to investigate these essential enzymes and new inhibitors. The new techniques covered in the chapters of this book include studying enzymes in vitro, in cells, and in animal models through proteomics, genomics, and structural biology. ... Leer más

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  • Golgi

    Methods and Protocols

    Series series Springer Protocols
    This volume provides readers with a collection of new and classical methods, techniques, and applications used to address enduring questions about the structure and functions of the Golgi complex. The chapters in this volume cover diverse topics ranging from model systems; live and fixed cell imaging techniques; in vitro biochemical reconstitution systems; and specific methods developed to study ... Leer más

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